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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
 

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Article DOI: 10.3791/59543-v • 05:50 min • April 10th, 2019
April 10th, 2019 •
1,2, 2,3
1Department of Biology, University of Maryland, 2Department of Cell Biology and Molecular Genetics, University of Maryland, 3Institute for Bioscience and Biotechnology Research, University of Maryland

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This protocol describes an in vivo phagocytosis assay in adult Drosophila melanogaster to quantify phagocyte recognition and clearance of microbial infections.

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In Vivo Phagocytosis Assay Cellular Immune Response Fruit Fly (Drosophila Melanogaster) Genetic Screens Genome-wide Association Studies Novel Genes Phagocytosis Regulation Adult Blood Cells Pathogen Recognition Uptake Clearance Thin-wall Glass Capillaries Microscope Micrometer Needle Puller Fine-point Stainless-steel Tweezers Tip Diameter Liquid Volume Measurement Sterile 5% Food Coloring In PBS Mineral Oil Stage Micrometer Particles Injector Nozzle Fly Pad Anesthetized Flies Abdomen Injection
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