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Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

DOI:
10.3791/59543-v

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05:50 min

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April 10, 2019

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,
1Department of Biology,University of Maryland, 2Department of Cell Biology and Molecular Genetics,University of Maryland, 3Institute for Bioscience and Biotechnology Research,University of Maryland

Capitoli

  • 00:04Titolo
  • 00:30Fly Injection
  • 02:32Fly Imaging and Fluorescence Quantification and Normalization
  • 03:59Results: Representative In Vivo Phagocytosis Assay Analyses
  • 05:18Conclusion

Summary

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This protocol describes an in vivo phagocytosis assay in adult Drosophila melanogaster to quantify phagocyte recognition and clearance of microbial infections.

Tags

PhagocytosisDrosophila MelanogasterIn Vivo AssayImmune ResponseCellular ImmunityGenetic ScreenGenome-wide Association StudyPathogen RecognitionPathogen ClearanceTrypan BlueFluorescence MicroscopyImaging Analysis

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