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Immunology and Infection

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JoVE Journal Immunology and Infection
An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
 

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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This protocol describes an in vitro batch-culture fermentation system of human fecal microbiota, using inulin (a well-known prebiotic and one of the most widely studied microbiota modulators) to demonstrate the use of this system in estimating effects of specific interventions on fecal microbiota composition and metabolic activities.

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In Vitro Batch-culture Model Human Fecal Microbiota Gut Microbiome Gut Microbiome Modulators Probiotics Prebiotics Diets Drugs Health Ailments Screening System Interventions Host Health Protocol Laboratories Aseptic Conditions Anaerobic Conditions Fecal Specimen Sample Storage Aliquot Prepping Pipetting Accuracy Reproducibility Culture Building Short-chain Fatty Acids Microbiome Determination
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