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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model
 

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

Article DOI: 10.3791/54819-v • 05:03 min • December 14th, 2016
December 14th, 2016 •
1, 2, 3, 4
1Physiology and Experimental Medicine, Hospital for Sick Children, 2Department of Clinical Microbiology, Royal College of Surgeons in Ireland, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Pediatrics, University of Toronto

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This protocol describes the visualization of biofilm development following exposure to host-factors using a slide chamber model. This model allows for direct visualization of biofilm development as well as analysis of biofilm parameters using computer software programs.

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Visualize Quantify Sputum Antibiotics Biofilm Development Chambered Coverglass Model Efficacy Bacterial Biofilms Methodology Experiment Sample Volume Phosphate-buffered Saline PBS Transfer Pipette Vortex Microcentrifuge Tubes Centrifugation Supernatant Pellet Filter Sterilize 0.22 Micron Filter Culture LB Media Optical Density
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