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Murine Fecal Isolation and Microbiota Transplantation
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07:32 min
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May 26, 2023
DOI:
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics,Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics,Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology,Vanderbilt University Medical Center
Summary
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The goal here is to outline a protocol to investigate the mechanisms of dysbiosis in cardiovascular disease. This paper discusses how to aseptically collect and transplant murine fecal samples, isolate intestines, and use the "Swiss-roll" method, followed by immunostaining techniques to interrogate changes in the gastrointestinal tract.
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Ishimwe, J. A., Zhong, J., Kon, V., Kirabo, A. Murine Fecal Isolation and Microbiota Transplantation. J. Vis. Exp. (195), e64310, doi:10.3791/64310 (2023).
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