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الاصطناعي RNA بولميراز الثاني استطالة المجمعات لتشريح شارك في النسخ RNA معالجة الأحداث

JoVE Journal
Genética

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JoVE Journal Genética

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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الاصطناعي RNA بولميراز الثاني استطالة المجمعات لتشريح شارك في النسخ RNA معالجة الأحداث

9,539 Views

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10:59 min

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May 13, 2019

DOI:

10.3791/59497-v

DOI:

10.3791/59497-v

•

10:59 min

May 13, 2019

•

7 Views

Melvin Noe Gonzalez, Joan W. Conaway², Ronald C. Conaway²

¹Stowers Institute for Medical Research, ²Department of Biochemistry and Molecular Biology,Kansas University Medical Center

Tags

RNA Polymerase II Elongation Complex Co-transcriptional RNA Processing Immobilization Magnetic Beads DNA Oligo Biotin Wash Buffer Annealing Thermal Cycler RNA-DNA Hybrid RNA Polymerase II Buffer

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