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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
 

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Article DOI: 10.3791/59448-v • 07:34 min • August 22nd, 2019
August 22nd, 2019 •
1,2,3, 2,3, 2,3, 1,2,3
1Department of Chemical and Biomolecular Engineering, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Pharmacology, University of South Alabama

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Spectral imaging has become a reliable solution for identification and separation of multiple fluorescence signals in a single sample and can readily distinguish signals of interest from background or autofluorescence. Excitation-scanning hyperspectral imaging improves on this technique by decreasing the necessary image acquisition time while simultaneously increasing the signal-to-noise ratio.

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Excitation-scanning Hyperspectral Imaging Microscopy Discriminate Fluorescence Signals Fluorescent Signals Traditional Methods Increase Speed Improve Sensitivity Illumination Sources Supercontinuum Laser Spinning Disk Laser Scanning Confocal Microscope Systems Excitation Peak Wavelength Inverted Fluorescence Microscope Camera Exposure Time Nanometers Live View Auto Intensity Viewing Range Button Focus Knobs Sample Position
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