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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
JoVE Journal
Engenharia
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JoVE Journal Engenharia
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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09:57 min

July 25, 2022

DOI:

09:57 min
July 25, 2022

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Summary

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We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

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