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原位杂交的色原性杂交是HPV相关头颈部癌症诊断的工具
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JoVE Journal Genética
Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
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原位杂交的色原性杂交是HPV相关头颈部癌症诊断的工具

DOI:
10.3791/59422-v

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06:57 min

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June 14, 2019

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, , , , , , , , ,
1Department of Pathology, Georges Pompidou European Hospital, Assistance publique – Hôpitaux de Paris (APHP),Paris Descartes University, 2Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, 3Laboratory of Immunology, Georges Pompidou European Hospital, APHP,Paris Descartes University

Capítulos

  • 00:04Título
  • 00:49Preparation of 1x Target Retrieval Reagent
  • 01:22Tissue Pretreatment
  • 03:12Hybridization
  • 04:49Signal Detection with 3,3’-diaminobenzidine
  • 05:45Results: “Low” and “High” RNA CISH Staining
  • 06:32Conclusion

Summary

Tadução automática

Tadução automática

人皮疹病毒(HPV)RNA原位染色体杂交被认为是肿瘤内活性人皮疹病毒感染检测的黄金标准之一。它允许通过定位和半定量评估其信号来可视化HPV E6-E7 mRNA表达。

Tags

Chromogenic In Situ HybridizationHPV RNA CISHHPV-related Head And Neck CancerActive HPV InfectionOropharyngeal Squamous Cell CarcinomaCervical NeoplasiaTarget Retrieval ReagentHydrogen PeroxideProtease DigestionHybridization Oven

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