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脓毒症诱导免疫抑制的切卡结扎和穿刺和鼻内感染双重模型的设计
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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脓毒症诱导免疫抑制的切卡结扎和穿刺和鼻内感染双重模型的设计

DOI:
10.3791/59386-v

•

07:30 min

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June 15, 2019

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, , , , ,
1West China School of Basic Medical Sciences & Forensic Medicine,Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences,University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital,Army Medical University

Capítulos

  • 00:00Título
  • 00:12Cecal Ligation and Puncture (CLP)
  • 03:29Secondary Intranasal Infection with Staphylococcus aureus
  • 05:52Resultados
  • 06:59Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了在免疫抑制条件下测量继发医院感染的传染性结果的技术,首先通过建立结扎/穿刺小鼠,然后挑战他们与鼻内感染创建一个临床相关的免疫抑制败血症模型。

Tags

Cecal LigationCecal PunctureSepsisImmunosuppressionMouse ModelIntranasal InfectionStaphylococcus AureusPneumonia

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