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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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07:30 min
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June 15, 2019
DOI:
1West China School of Basic Medical Sciences & Forensic Medicine,Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences,University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital,Army Medical University
Summary
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This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.
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Wang, Z., Pu, Q., Lin, P., Li, C., Jiang, J., Wu, M. Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression. J. Vis. Exp. (148), e59386, doi:10.3791/59386 (2019).
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