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用噻唑橙代替溴代或替代染料进行 DNA 电泳
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Bioquímica
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JoVE Journal Bioquímica
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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用噻唑橙代替溴代或替代染料进行 DNA 电泳

DOI:
10.3791/59341-v

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04:18 min

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March 31, 2019

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, ,
1Department of Molecular Biology and Chemistry,Christopher Newport University

Capítulos

  • 00:04Título
  • 00:29Preparing the Gel
  • 01:29Loading and Running the Gel
  • 02:20Visualization of Thiazole Orange Agarose Gel
  • 03:08Image Capture
  • 03:28Results: DNA Staining with Thiazole Orange in Electrophoresis Experiments
  • 03:58Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个协议, 使用噻唑橙检测 DNA 的凝胶电泳实验。使用噻唑橙可以消除溴化乙酯, 并且可以使用紫外或蓝光进行荧光检测。

Tags

Keywords: DNA ElectrophoresisThiazole OrangeEthidium BromideAlternative DyesUV LightBlue LightAgarose GelTAE BufferTBE BufferDNA DetectionDNA VisualizationDNA ExtractionDownstream Experiments

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