Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
13,452 Views
•
04:18 min
•
March 31, 2019
DOI:
Summary
Automatically generated
Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.
Read Article
Cite this Article
O’Neil, C. S., Beach, J. L., Gruber, T. D. DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes. J. Vis. Exp. (145), e59341, doi:10.3791/59341 (2019).
Copy