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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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04:18 min
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March 31, 2019
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Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.
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O’Neil, C. S., Beach, J. L., Gruber, T. D. DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes. J. Vis. Exp. (145), e59341, doi:10.3791/59341 (2019).
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