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JoVE Journal Biochemistry
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
 

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Article DOI: 10.3791/59331-v • 10:41 min • June 24th, 2019
June 24th, 2019 •
1,2, 1, 1, 1, 1, 1
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, 2UCLA Institute for Technology Advancement (Suzhou)

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This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.

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Amino Acid Overproducers Rare-codon-rich Markers Alternative Method Analog-based Approach Accuracy Sensitivity High Throughput Microorganisms Molecular Experimental Operations Straining Stringencies Visual Demonstration Screening System Vector Assembly Mix Transformation Super Optimal Broth With Catabolite Repression Medium LB Agar Medium Plasmid Isolation
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