ジャーナル
/
生化学
/
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers

DOI:
10.3791/59331-v

•

10:41 min

•

June 24, 2019

•

, , , , ,
1Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences,Beijing Institute of Technology, 2UCLA Institute for Technology Advancement (Suzhou)

章

  • 00:04標題
  • 01:02Construction of the Plasmids Expressing the Rare-codon-rich Marker Genes
  • 01:50Optimizing the Selection Conditions
  • 03:23Optimizing the Screening Conditions
  • 04:23Identification of the Amino Acid Overproducers
  • 07:51Results: Identifying Amino Acid Overproducers
  • 09:43Conclusion

概要

自動翻訳

自動翻訳

This study presents an alternative strategy to the conventional toxic analog-based method in identifying amino acid overproducers by using rare-codon-rich markers to achieve accuracy, sensitivity, and high-throughput simultaneously.

タグ

Amino Acid OverproducersRare-codon-rich MarkersAnalog-based ApproachHigh ThroughputTranslation Of Rare CodonsMolecular Experimental OperationsSelection StringenciesVisual DemonstrationSelection And Screening SystemKanRRare Codon CTAWild-type Selection Marker GeneRare-codon-rich DerivativeOD600L-leucine

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria

Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)

Read Article