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Imaging of Extracellular Vesicles by Atomic Force Microscopy
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JoVE Journal Bioquímica
Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

12,590 Views

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10:11 min

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September 11, 2019

DOI:

10.3791/59254-v

DOI:
10.3791/59254-v

•

10:11 min
September 11, 2019

•

38 Views
,
1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Summary

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A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

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Cite this Article

Skliar, M., Chernyshev, V. S. Imaging of Extracellular Vesicles by Atomic Force Microscopy. J. Vis. Exp. (151), e59254, doi:10.3791/59254 (2019).

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