Journal
/
Biochimie
/
Imaging of Extracellular Vesicles by Atomic Force Microscopy
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

12,557 Views

•

10:11 min

•

September 11, 2019

DOI:

10.3791/59254-v

DOI:
10.3791/59254-v

•

10:11 min
September 11, 2019

•

38 Views
,
1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Summary

Automatically generated

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

Explore More Videos

Extracellular VesiclesAtomic Force MicroscopyAFM ImagingElectrostatic ImmobilizationMica SurfaceHydrated SamplesDesiccated SamplesCryoTEMNanoparticle Tracking Analysis

Read Article

Cite this Article

Skliar, M., Chernyshev, V. S. Imaging of Extracellular Vesicles by Atomic Force Microscopy. J. Vis. Exp. (151), e59254, doi:10.3791/59254 (2019).

Download .ris file

Copy

Share Video

.

Copy