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Breath Collection from Children for Disease Biomarker Discovery
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06:09 min
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February 14, 2019
DOI:
1Department of Pediatrics,Washington University School of Medicine, 2Cell Biology & Physiology,Washington University School of Medicine, 3Division of Gastroenterology, Hepatology and Nutrition,Washington University School of Medicine, 4Department of Molecular Microbiology,Washington University School of Medicine
Summary
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This protocol describes a simple method for the acquisition of breath samples from children. Briefly, samples of mixed air are pre-concentrated in sorbent tubes prior to gas chromatography-mass spectrometry analysis. Breath biomarkers of infectious and non-infectious diseases can be identified using this breath collection method.
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Berna, A. Z., DeBosch, B., Stoll, J., Odom John, A. R. Breath Collection from Children for Disease Biomarker Discovery. J. Vis. Exp. (144), e59217, doi:10.3791/59217 (2019).
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