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Breath Collection from Children for Disease Biomarker Discovery
1Department of Pediatrics,Washington University School of Medicine, 2Cell Biology & Physiology,Washington University School of Medicine, 3Division of Gastroenterology, Hepatology and Nutrition,Washington University School of Medicine, 4Department of Molecular Microbiology,Washington University School of Medicine
Chapters
- 00:04Title
- 00:54Breath Sample Equipment Assembly
- 01:42Breath Collection
- 02:46Ambient Air Collection
- 03:35Thermal Desorption Tube Breath Transfer
- 04:33Results: Representative Exhaled Breath Volatile Assessment
- 05:39Conclusion
This protocol describes a simple method for the acquisition of breath samples from children. Briefly, samples of mixed air are pre-concentrated in sorbent tubes prior to gas chromatography-mass spectrometry analysis. Breath biomarkers of infectious and non-infectious diseases can be identified using this breath collection method.
