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原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq 检测

JoVE Journal
Genética

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JoVE Journal Genética

ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes

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原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq 检测

12,266 Views

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08:36 min

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March 22, 2019

DOI:

10.3791/59120-v

DOI:

10.3791/59120-v

•

08:36 min

March 22, 2019

•

4 Views

Hannah D. Rickner, Sheng-Yong Niu², Christine S. Cheng³

¹Department of Biology,Boston University, ²Department of Computer Science and Engineering,University of California San Diego, ³Bioinformatics Program,Boston University

Tags

ATAC-seq Mitochondrial DNA Contamination CD4+ T Lymphocytes Cell Activation Cell Selection Cell Isolation Cell Counting Magnetic Beads Cell Culture Cell Stimulation

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