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Immunology and Infection

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JoVE Journal Immunology and Infection
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
 

A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

Article DOI: 10.3791/58961-v • 10:44 min • June 2nd, 2019
June 2nd, 2019 •
1, 1, 2, 1,2
1Department of Emergency Medicine, University of Mississippi Medical Center, 2Department of Experimental Therapeutics and Pharmacology, University of Mississippi Medical Center

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Here, we provide a detailed methodology to isolate and assess cytotoxic function of natural killer cells from placentas by a colorimetric plate assay. The reduced uterine perfusion pressure rat model of placental ischemia was chosen to demonstrate the antibody-mediated isolation and assessment of the cytotoxic function of natural killer cells.

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Plate-based Cytotoxicity Assay Rat Placental Natural Killer Cell Cytolytic Function Cytotoxic Activity Cell Types Simple Method Inexpensive Method No Specialized Equipment Hazardous Materials CD8 Plus Cytolytic Cells Spontaneous Death Of Cells Optimization Of Target To Effector Cell Ratio Literature Search Isolation Of Mononuclear Cells Assay Plate Setup Chelsea Jekelley Technician RPMI FBS Petri Dish
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