Journal
/
Imunologia e Infecção
/
双氟铬流式细胞仪对七种免疫细胞子集的判别
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

双氟铬流式细胞仪对七种免疫细胞子集的判别

13,467 Views

•

10:58 min

•

March 05, 2019

DOI:

10.3791/58955-v

DOI:
10.3791/58955-v

•

10:58 min
March 05, 2019

•

6 Views
,
1Oncology Research,Medimmune, LLC, 2Division of Rheumatology,Johns Hopkins University School of Medicine

Summary

Automatically generated

在这里, 我们提出了一个流式细胞仪协议, 以识别 cd4+和 cd8 + t 细胞, γ t细胞, b 细胞, nk 细胞和单核细胞在人外周血中只使用两个荧光色素, 而不是七个。通过这种方法, 可以在大多数流式细胞仪上记录五个附加标记。

Explore More Videos

Flow CytometryImmune Cell SubsetsTwo-fluorochromePBMCDensity GradientLive/dead StainingAntibody PanelDeep Immunophenotyping

Read Article

Cite this Article

Torchia, M. L. G., Cimbro, R. Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry. J. Vis. Exp. (145), e58955, doi:10.3791/58955 (2019).

Download .ris file

Copy

Share Video

.

Copy