/
/
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,480 Views
•
10:58 min
•
March 05, 2019
DOI:
Summary
Automatically generated
Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
Read Article
Cite this Article
Torchia, M. L. G., Cimbro, R. Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry. J. Vis. Exp. (145), e58955, doi:10.3791/58955 (2019).
Copy