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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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March 05, 2019
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Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.
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Torchia, M. L. G., Cimbro, R. Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry. J. Vis. Exp. (145), e58955, doi:10.3791/58955 (2019).
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