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Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
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Neurociência
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JoVE Journal Neurociência
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

DOI:
10.3791/58940-v

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09:18 min

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January 29, 2019

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, ,
1Institute of Anatomy and Embryology,University Medical Center Göttingen

Capítulos

  • 00:04Título
  • 00:55Sample Preparation
  • 02:28Immunofluorescence
  • 06:08Analysis of Confocal Images
  • 07:11Results: Determining the Abundance of Proteins of Interest Across the Brain
  • 08:43Conclusion

Summary

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Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.

Tags

Keywords: ImmunofluorescenceConfocal MicroscopyProtein DistributionHeterogeneityMouse BrainCryo-protectionCryosectioningBrain SlicesImmunostainingQuantification

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