Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.
Wallrafen, R., Dresbach, T., Viotti, J. S. Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence. J. Vis. Exp. (143), e58940, doi:10.3791/58940 (2019).