Journal
/
Neurowissenschaften
/
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

DOI:
10.3791/58940-v

•

09:18 min

•

January 29, 2019

•

, ,
1Institute of Anatomy and Embryology,University Medical Center Göttingen

Kapitel

  • 00:04Titel
  • 00:55Sample Preparation
  • 02:28Immunofluorescence
  • 06:08Analysis of Confocal Images
  • 07:11Results: Determining the Abundance of Proteins of Interest Across the Brain
  • 08:43Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.

Tags

ImmunofluorescenceConfocal MicroscopyProtein DistributionHeterogeneityMouse BrainCryo-protectionCryosectioningBrain SlicesImmunostainingQuantification

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Use of Synaptic Zinc Histochemistry to Reveal Different Regions and Laminae in the Developing and Adult Brain

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Read Article