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头脊髓和薄片体外制备心律失常活性

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

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头脊髓和薄片体外制备心律失常活性

DOI:

10.3791/58870-v

•

06:32 min

•

March 23, 2019

•

Samantha B. Palahnuk², Jonathan A. Abdala, Vadim V. Gospodarev, Christopher G. Wilson

¹Center for Perinatal Biology, Department of Basic Sciences,Loma Linda University, ²Department of Biology,The College of New Jersey, ³Department of Neurosurgery,Loma Linda University

Capítulos

00:04Título
00:41Neuraxis Isolation
03:59Histological Preparation
05:15Results: Representative Recordings from En Bloc and Slice Preparations
05:52Conclusion

Summary

Tadução automática

该方案既直观地传达脑干脊髓的准备, 并阐明脑干横向切片的准备工作, 一个全面的逐步的方式。它的目的是提高重现性, 并提高获得可行的, 持久的, 有节奏的活性切片记录神经输出从脑干的呼吸区域的可能性。

Tags

Keywords: Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord Thin Slice Dissection Mirian Medullary Respiratory Network Electrophysiology Neural Control Circuit Breathing Rhythms Genetically Modified Rodents Dorsal Laminectomy Ventral Laminectomy Pons Medulla Spinal Cord

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