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Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
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Neurociência
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JoVE Journal Neurociência
Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

DOI:
10.3791/58870-v

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06:32 min

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March 23, 2019

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, , ,
1Center for Perinatal Biology, Department of Basic Sciences,Loma Linda University, 2Department of Biology,The College of New Jersey, 3Department of Neurosurgery,Loma Linda University

Capítulos

  • 00:04Título
  • 00:41Neuraxis Isolation
  • 03:59Histological Preparation
  • 05:15Results: Representative Recordings from En Bloc and Slice Preparations
  • 05:52Conclusion

Summary

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This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.

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Keywords: Rhythmically-activeIn VitroNeonatalRodentBrainstem-spinal CordThin SliceDissectionMirian Medullary Respiratory NetworkElectrophysiologyNeural Control CircuitBreathing RhythmsGenetically Modified RodentsDorsal LaminectomyVentral LaminectomyPonsMedullaSpinal Cord

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