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在体外显示砷对皮肤细胞迁移影响的划痕测定
JoVE Journal
Ambiente
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JoVE Journal Ambiente
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
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在体外显示砷对皮肤细胞迁移影响的划痕测定

DOI:
10.3791/58838-v

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09:24 min

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February 23, 2019

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, , , ,
1Department of Biological Sciences,Northern Arizona University, 2Department of Mechanical Engineering,Northern Arizona University, 3Department of Chemistry and Biochemistry,Northern Arizona University, 4Center for Bioengineering Innovation,Northern Arizona University

Capítulos

  • 00:04Título
  • 01:05Human Dermal Fibroblasts Seeding in a T75 Flask
  • 02:37Cell Counting and Subculture into 12-Well Tissue Culture Plate
  • 06:11Scratch Assay Technique and Arsenic Contamination
  • 07:44Results: Analysis of the Scratch Assay
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

本研究的重点是伤口愈合的体外模型 (划痕检测), 作为一种机制, 以确定环境污染物, 如砷如何影响细胞迁移。结果表明, 这种体外检测在体内实验之前为细胞迁移的变化提供了快速和早期的迹象。

Tags

Keywords: In Vitro Scratch AssayCell MigrationWound HealingArsenicSkin CellsCell CultureCell Migration AssayCell MonolayerCell LineCell SuspensionCell Density

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