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In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

22,369 Views

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09:24 min

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February 23, 2019

DOI:

10.3791/58838-v

DOI:
10.3791/58838-v

•

09:24 min
February 23, 2019

•

25 Views
, , , ,
1Department of Biological Sciences,Northern Arizona University, 2Department of Mechanical Engineering,Northern Arizona University, 3Department of Chemistry and Biochemistry,Northern Arizona University, 4Center for Bioengineering Innovation,Northern Arizona University

Summary

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This study focuses on an in vitro model of wound healing (scratch assay) as a mechanism for determining how environmental contaminants such as arsenic influence cellular migration. The results demonstrate that this in vitro assay provides rapid and early indications of changes to cellular migration prior to in vivo experimentation.

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Cite this Article

Pinto, B. I., Cruz, N. D., Lujan, O. R., Propper, C. R., Kellar, R. S. In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration. J. Vis. Exp. (144), e58838, doi:10.3791/58838 (2019).

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