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In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
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In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

DOI:
10.3791/58838-v

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09:24 min

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February 23, 2019

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, , , ,
1Department of Biological Sciences,Northern Arizona University, 2Department of Mechanical Engineering,Northern Arizona University, 3Department of Chemistry and Biochemistry,Northern Arizona University, 4Center for Bioengineering Innovation,Northern Arizona University

Chapters

  • 00:04Title
  • 01:05Human Dermal Fibroblasts Seeding in a T75 Flask
  • 02:37Cell Counting and Subculture into 12-Well Tissue Culture Plate
  • 06:11Scratch Assay Technique and Arsenic Contamination
  • 07:44Results: Analysis of the Scratch Assay
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

This study focuses on an in vitro model of wound healing (scratch assay) as a mechanism for determining how environmental contaminants such as arsenic influence cellular migration. The results demonstrate that this in vitro assay provides rapid and early indications of changes to cellular migration prior to in vivo experimentation.

Tags

Keywords: In Vitro Scratch AssayCell MigrationWound HealingArsenicSkin CellsCell CultureCell Migration AssayCell MonolayerCell LineCell SuspensionCell Density

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