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Imunologia e Infecção
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Isolering af enkelt intracellulære bakterielle samfund genereret fra en Murine Model af urinvejsinfektion for Downstream encellede analyse
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Isolering af enkelt intracellulære bakterielle samfund genereret fra en Murine Model af urinvejsinfektion for Downstream encellede analyse

DOI:
10.3791/58829-v

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07:34 min

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April 16, 2019

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, , , , ,
1Infectious Diseases Group,Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering,National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine,National University of Singapore

Capítulos

  • 00:04Título
  • 00:41Glass Capillary Preparation
  • 02:06Bladder Epithelial Cell Harvest
  • 03:53Intracellular Bacterial Community (IBC) Isolation
  • 05:18Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
  • 06:30Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en simpel metode til at isolere enkelt, inficeret blæren epitel celler fra en murine model af urinvejsinfektion.

Tags

Keywords: Intracellular Bacterial CommunitiesIBCsUrinary Tract InfectionUTIMouse BladderMouth MicropipettingGlass Capillary TubeBladder Epithelial CellsSingle-cell Analysis

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