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在 vivosilk/aav 薄膜在光基因表达中的靶向表达
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JoVE Journal Neurociência
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
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在 vivosilk/aav 薄膜在光基因表达中的靶向表达

DOI:
10.3791/58728-v

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06:11 min

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February 26, 2019

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, ,
1Vollum Institute,Oregon Health and Science University, 2Harvard Medical School

Capítulos

  • 00:04Conclusion
  • 00:35Mix Aqueous Silk with AAV Expression Vectors and Apply Silk/AAV Film to Devices
  • 04:11Results: Targeted Delivery of Expression Vectors Using Silk/AAV Optical Fibers
  • 05:24Conclusion

Summary

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Tadução automática

在这里, 我们提出了一种方法, 提供病毒表达载体到大脑使用丝素薄膜。这种方法允许使用 silk/aav 涂层光纤、锥形光纤和颅窗定向表达向量的传递。

Tags

Keywords: OptogeneticsIn VivoTargeted ExpressionSilk/AAV FilmsNeuronal EnsemblesSensory ProcessingCognitive ProcessingChan RhodopsinGCaMPOptical ImplantsAAVSilk FibroinStereotaxic ApparatusMicroinjectorOptical FiberVacuum Desiccation

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