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In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
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Neuroscienze
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JoVE Journal Neuroscienze
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

DOI:
10.3791/58728-v

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06:11 min

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February 26, 2019

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, ,
1Vollum Institute,Oregon Health and Science University, 2Harvard Medical School

Capitoli

  • 00:04Conclusion
  • 00:35Mix Aqueous Silk with AAV Expression Vectors and Apply Silk/AAV Film to Devices
  • 04:11Results: Targeted Delivery of Expression Vectors Using Silk/AAV Optical Fibers
  • 05:24Conclusion

Summary

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Here, we present a method for delivering viral expression vectors into the brain using silk fibroin films. This method allows targeted delivery of expression vectors using silk/AAV coated optical fibers, tapered optical fibers, and cranial windows.

Tags

Keywords: OptogeneticsIn VivoTargeted ExpressionSilk/AAV FilmsNeuronal EnsemblesSensory ProcessingCognitive ProcessingChan RhodopsinGCaMPOptical ImplantsAAVSilk FibroinStereotaxic ApparatusMicroinjectorOptical FiberVacuum Desiccation

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