Journal
/
Neurociencias
/
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

DOI:
10.3791/58728-v

•

06:11 min

•

February 26, 2019

•

, ,
1Vollum Institute,Oregon Health and Science University, 2Harvard Medical School

Capítulos

  • 00:04Conclusion
  • 00:35Mix Aqueous Silk with AAV Expression Vectors and Apply Silk/AAV Film to Devices
  • 04:11Results: Targeted Delivery of Expression Vectors Using Silk/AAV Optical Fibers
  • 05:24Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a method for delivering viral expression vectors into the brain using silk fibroin films. This method allows targeted delivery of expression vectors using silk/AAV coated optical fibers, tapered optical fibers, and cranial windows.

Tags

Keywords: OptogeneticsIn VivoTargeted ExpressionSilk/AAV FilmsNeuronal EnsemblesSensory ProcessingCognitive ProcessingChan RhodopsinGCaMPOptical ImplantsAAVSilk FibroinStereotaxic ApparatusMicroinjectorOptical FiberVacuum Desiccation

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/58728?article_type=v

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
check_url/es/58728?article_type=v

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
check_url/es/58728?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/es/58728?article_type=v

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
check_url/es/58728?article_type=v

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
check_url/es/58728?article_type=v

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
check_url/es/58728?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/es/58728?article_type=v

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
check_url/es/58728?article_type=v

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
check_url/es/58728?article_type=v

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Read Article