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RNA-baserte omprogrammering av menneskelig primære fibroblaster til indusert Pluripotent stamceller
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1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus
Capítulos
- 00:04Título
- 00:45Reagents Preparation
- 02:01Plating Fibroblasts for Reprogramming: Day 0
- 05:09Initiation of Reprogramming: Day 1
- 09:28Results: Efficient Reprogramming of Human Primary Fibroblasts into iPSCs
- 11:16Conclusion
Her beskriver vi en klinisk relevant, høy effektivitet, mater-fri metode for å omprogrammere menneskelig primære fibroblaster i indusert pluripotent stilk celler med endrede mRNAs koding omprogrammering faktorer og moden microRNA-367/302 etterligner. Også inkludert er metoder for å vurdere omprogrammering effektivitet, utvide klonal iPSC koloniene, og Bekreft uttrykk for pluripotency markøren TRA-1-60.
