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基于 rna 的人原代成纤维细胞重组成诱导多能干细胞
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11:38 min
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November 26, 2018
DOI:
1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus
Summary
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在这里, 我们描述了一种临床相关的, 高效率, 无微弱的方法, 重新编程人类的原代成纤维细胞到诱导多能干细胞使用修改 mrna 编码重编程因子和成熟的微 rna-367/302 模拟。还包括评估重新编程效率、扩大克隆 ipsc 菌落和确认多能标记 tra-1-60 的表达的方法。
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Cite this Article
McGrath, P. S., Diette, N., Kogut, I., Bilousova, G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J. Vis. Exp. (141), e58687, doi:10.3791/58687 (2018).
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