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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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11:38 min
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November 26, 2018
DOI:
1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus
Summary
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Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker TRA-1-60.
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McGrath, P. S., Diette, N., Kogut, I., Bilousova, G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J. Vis. Exp. (141), e58687, doi:10.3791/58687 (2018).
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