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인간의 기본 섬유 아 세포의 유도 만능 줄기 세포로 재프로그래밍 RNA 기반
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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인간의 기본 섬유 아 세포의 유도 만능 줄기 세포로 재프로그래밍 RNA 기반

9,865 Views

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11:38 min

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November 26, 2018

DOI:

10.3791/58687-v

DOI:
10.3791/58687-v

•

11:38 min
November 26, 2018

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2 Views
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1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus

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RNA-based ReprogrammingInduced Pluripotent Stem CellsIPSCsPrimary FibroblastsTransfection EfficiencyPH AdjustmentLaminin 521Fibroblast Expansion MediumPlating MediumFGFB18R

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McGrath, P. S., Diette, N., Kogut, I., Bilousova, G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J. Vis. Exp. (141), e58687, doi:10.3791/58687 (2018).

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