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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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発生生物学
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JoVE Journal 発生生物学
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

DOI:
10.3791/58687-v

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11:38 min

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November 26, 2018

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, , ,
1皮膚学部,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus

章

  • 00:04標題
  • 00:45Reagents Preparation
  • 02:01Plating Fibroblasts for Reprogramming: Day 0
  • 05:09Initiation of Reprogramming: Day 1
  • 09:28Results: Efficient Reprogramming of Human Primary Fibroblasts into iPSCs
  • 11:16Conclusion

概要

自動翻訳

自動翻訳

Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker TRA-1-60.

タグ

RNA-based ReprogrammingInduced Pluripotent Stem CellsIPSCsPrimary FibroblastsTransfection EfficiencyPH AdjustmentLaminin 521Fibroblast Expansion MediumPlating MediumFGFB18R

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