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מבוסס-RNA תכנות מחדש של האנושי Fibroblasts הראשי לתוך תאי גזע Pluripotent המושרה
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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מבוסס-RNA תכנות מחדש של האנושי Fibroblasts הראשי לתוך תאי גזע Pluripotent המושרה

9,865 Views

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11:38 min

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November 26, 2018

DOI:

10.3791/58687-v

DOI:
10.3791/58687-v

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11:38 min
November 26, 2018

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2 Views
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1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus

Tags

RNA-based ReprogrammingInduced Pluripotent Stem CellsIPSCsPrimary FibroblastsTransfection EfficiencyPH AdjustmentLaminin 521Fibroblast Expansion MediumPlating MediumFGFB18R

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Cite this Article

McGrath, P. S., Diette, N., Kogut, I., Bilousova, G. RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. J. Vis. Exp. (141), e58687, doi:10.3791/58687 (2018).

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