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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

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05:30 min

January 23, 2019

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05:30 min
January 23, 2019

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Summary

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This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

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