Journal
/
Bioengenharia
/
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

13,064 Views

•

05:30 min

•

January 23, 2019

DOI:

10.3791/58589-v

DOI:
10.3791/58589-v

•

05:30 min
January 23, 2019

•

9 Views
, , , ,
1bisy e.U., 2Institute of Molecular Biotechnology,Technical University of Graz

Summary

Automatically generated

This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

Explore More Videos

Pichia PastorisMethanolDe-repressionRecombinant Protein ExpressionShake Flask CultivationOnline MonitoringBiomass MeasurementBluetoothCell DensityGlycerol FeedExponential Growth Phase

Read Article

Cite this Article

Fischer, J. E., Hatzl, A., Weninger, A., Schmid, C., Glieder, A. Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies. J. Vis. Exp. (143), e58589, doi:10.3791/58589 (2019).

Download .ris file

Copy

Share Video

.

Copy