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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

13,037 Views

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05:30 min

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January 23, 2019

DOI:

10.3791/58589-v

DOI:
10.3791/58589-v

•

05:30 min
January 23, 2019

•

9 Views
, , , ,
1bisy e.U., 2Institute of Molecular Biotechnology,Technical University of Graz

Summary

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This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

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Cite this Article

Fischer, J. E., Hatzl, A., Weninger, A., Schmid, C., Glieder, A. Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies. J. Vis. Exp. (143), e58589, doi:10.3791/58589 (2019).

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