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使用培养的果蝇卵室延时电影对局部细胞和组织尺度的actomyosin动力学的分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
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使用培养的果蝇卵室延时电影对局部细胞和组织尺度的actomyosin动力学的分析

DOI:
10.3791/58587-v

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10:45 min

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June 03, 2019

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, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden

Capítulos

  • 00:04Título
  • 00:57Cell Segmentation: Generation of a Cell Mask for Time Lapse Movies
  • 02:33Loading the Generated Cell Mask into Surface Manager
  • 03:36Analysis of Actomyosin Pulses in Surface Manager at Local Cellular Scale
  • 04:51Selective Surface Extraction of Curved Epithelial Tissues
  • 08:41Results: Behavior of Myosin II at the Cellular and Tissue Scale in a Drosophila Egg Chamber
  • 10:13Conclusion

Summary

Tadução automática

Tadução automática

该协议提供了一种基于斐济的、用户友好的方法,以及解释如何可靠地分析单个细胞和弯曲上皮组织中的actomyosin行为的直截了当的说明。无需编程技能即可遵循本教程;所有步骤均使用斐济的图形用户界面和相关插件以半交互式方式执行。

Tags

Actomyosin DynamicsDrosophila Egg ChambersTime-lapse MoviesCell SegmentationEpithelial CellsSurface ManagerFijiImage AnalysisCell MembraneCell Outline

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