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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

7,294 Views

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10:45 min

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June 03, 2019

DOI:

10.3791/58587-v

DOI:
10.3791/58587-v

•

10:45 min
June 03, 2019

•

2 Views
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1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden

Summary

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This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

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Cite this Article

Viktorinová, I., Haase, R., Pietzsch, T., Henry, I., Tomancak, P. Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers. J. Vis. Exp. (148), e58587, doi:10.3791/58587 (2019).

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