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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

DOI:
10.3791/58587-v

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10:45 min

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June 03, 2019

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, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden

Capítulos

  • 00:04Título
  • 00:57Cell Segmentation: Generation of a Cell Mask for Time Lapse Movies
  • 02:33Loading the Generated Cell Mask into Surface Manager
  • 03:36Analysis of Actomyosin Pulses in Surface Manager at Local Cellular Scale
  • 04:51Selective Surface Extraction of Curved Epithelial Tissues
  • 08:41Results: Behavior of Myosin II at the Cellular and Tissue Scale in a Drosophila Egg Chamber
  • 10:13Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

Tags

Keywords: Actomyosin DynamicsDrosophila Egg ChambersTime-lapse MoviesCell SegmentationEpithelial CellsSurface ManagerFijiImage AnalysisCell MembraneCell Outline

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