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iSCAT माइक्रोस्कोपी के माध्यम से कोशिकाओं रहने से स्रावित एकल प्रोटीन की लेबल-मुक्त इमेजिंग

JoVE Journal
Engenharia

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JoVE Journal Engenharia

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

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iSCAT माइक्रोस्कोपी के माध्यम से कोशिकाओं रहने से स्रावित एकल प्रोटीन की लेबल-मुक्त इमेजिंग

15,877 Views

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10:55 min

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November 20, 2018

DOI:

10.3791/58486-v

DOI:

10.3791/58486-v

•

10:55 min

November 20, 2018

•

18 Views

André Gemeinhardt, Matthew P. McDonald², Katharina König³, Michael Aigner, Andreas Mackensen, Vahid Sandoghdar³

¹Max Planck Institute for the Science of Light (MPL), ²Area of Scientific Learning,Milligan College, ³Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, ⁴Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

Tags

Label-free Imaging Single Proteins ISCAT Microscopy Biological Nanoparticles Extinction Cross Section Scattering Background Home-built Microscope LUZ Cells Molecular-level Probing Optical Setup Damped Optical Table High Numerical Aperture Objective Diode Laser Wide-field Lens Immersion Oil Glass Coverslip Anti-reflection Coated Beamsplitter

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