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ללא תווית הדמיה של יחיד חלבונים מופרש מתאי חי דרך iSCAT מיקרוסקופ
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November 20, 2018
DOI:
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
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Label-free ImagingSingle ProteinsISCAT MicroscopyBiological NanoparticlesExtinction Cross SectionScattering BackgroundHome-built MicroscopeLUZ CellsMolecular-level ProbingOptical SetupDamped Optical TableHigh Numerical Aperture ObjectiveDiode LaserWide-field LensImmersion OilGlass CoverslipAnti-reflection Coated Beamsplitter
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Gemeinhardt, A., McDonald, M. P., König, K., Aigner, M., Mackensen, A., Sandoghdar, V. Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy. J. Vis. Exp. (141), e58486, doi:10.3791/58486 (2018).
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